Affordable Luciferase Reporter Assay for Cell-Based High-Throughput Screening
نویسندگان
چکیده
منابع مشابه
Development of a high-throughput screening cancer cell-based luciferase refolding assay for identifying Hsp90 inhibitors.
The 90 kDa heat-shock protein (Hsp90) and other cochaperones allow for proper folding of nascent or misfolded polypeptides. Cancer cells exploit these chaperones by maintaining the stability of mutated and misfolded oncoproteins and allowing them to evade proteosomal degradation. Inhibiting Hsp90 is an attractive strategy for cancer therapy, as the concomitant degradation of multiple oncoprotei...
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ژورنال
عنوان ژورنال: Journal of Biomolecular Screening
سال: 2012
ISSN: 1087-0571,1552-454X
DOI: 10.1177/1087057112465184